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The Technology behind COLIKAT®
Defined Substrate Technology (DST) to simultaneously detect total coliforms and E.coli in water according to EN ISO 9302-8
COLIKAT RAPID®
COLIKAT RAPID®, utilizes a Defined Substrate Technology (DST) to simultaneously detect total coliform and Escherichia coli in water according to EN ISO 9308-2, providing the test results in 18 hours with no confirmation tests required.
COLIKAT RAPID® is available in Presence / Absence and Quantification test formats, using Enumeration trays and the Seal Applicator to support a wide range of water matrices and workflows.
The manufacturing, storage and quality control of COLIKAT RAPID® is carried out in an ISO 9001 certified Xebios Diagnostics facility in compliance with EN ISO 11133 and Good Manufacturing Practice (GMP). The quality assurance testing of COLIKAT RAPID® takes place in EN ISO 17025 accredited laboratory that complies with all the requirements of EN ISO 11133 and EN ISO 9308-2.
The Test Principle
COLIKAT RAPID® uses certain specific characteristics of both Escherichia coli and coliform bacteria for identification of the species. The test contains MUG and ONPG, two common nutritional indicators. Coliform bacteria use their enzyme β-Galactosidase to metabolize ONPG turning the sample yellow. Escherichia coli – other than most coliform bacteria – additionally has the enzyme β-Glucuronidase that enables it to hydrolyze MUG so that the sample additionally creates a fluorescence reaction under UV-light.
Most of non-coliform bacteria possess neither the β-Galactosidase nor the β-Glucuronidase. Therefore, they cannot transform MUG and ONPG and the test will not detect them. Yet, some non-coliform bacteria contain the enzymes. COLIKAT RAPID® therefore uses selective supplements that inhibit the growth of those bacteria. The combination of the test’s enzymes and selective supplements prevents some common problems of conventional culture methods such as Chromogen Coliform Agar CCA (EN ISO 9308-1):
- False positive results are prevented (for example for Aeromonas spec.)
- The growth of non-target organism is prohibited. This way non-target organisms cannot affect (or even inhibit) the growth of Escherichia coli and coliform bacteria.
The Instructions for Use: Presence/ Absence
COLIKAT RAPID® Instructions for use
Presence/ Absence: Add 1 bag of Colikat Rapid to a sample of 100 ml of water. Close the sample vessle and shake until the reagent dissolves.
For Presence/ Absence testing, incubate the closed vessel for 18 – 22 hours at 36 Degrees Celsius and read the result. The vessels should be at room temperature before incubation. If the sample turns yellow, coliforms are present. If it shows fluorescence under UV light, Escherichia coli is present.
The Instructions for Use: Quantification
Note: Step 1 and 1.2 as indicated in the Presence/ Absence test, must take place before Step 2 is uindertaken.
For quantitative testing pour the 100 ml sample into the COLIKAT Enumeration Tray. COLIKAT RAPID® turned yellow means coliforms are present. If it shows fluorescence under UV-light Escherichia coli is present.
Seal the COLIKAT Enumeration Tray with the COLIKAT Seal applicator or another enumeration tray sealing device.
Incubate the sample for 18 – 22 hours at 36 Degrees Celsius. Count the yellow wells if the COLIKAT Enumeration Tray (= coliforms). Count the yellow wells of the COLIKAT Enumeration Tray that shows fluorescence under UV-light (365nm) in a dark environment (= Escherichia coli).
Transform the count results to the number of CFU in the sample by using the MPN table in the appendix to EN ISO 9308 -2.
ISO 17994 Comparison Study & Quality
“The study clearly proved that the XEBIOS‘s COLIKAT RAPID® method is equivalent to the IDEXX‘s COLILERT-18/QUANTI-Tray® method for the enumeration of coliform bacteria.
For the target organism E. coli the study clearly proved that XEBIOS‘s COLIKAT RAPID® has a significantly higher recovery compared to IDEXX‘s reference method COLILERT- 18®/QUANTI-Tray®.”
The above quotation comes from: IWW Rheinisch-Westfälisches Institut für Wasser Beratungs- und Entwicklungsgesellschaft mbH, January 2021 [Final report of a comparison study for the evaluation of the equivalence of XEBIOS DIAGNOSTICS COLIKAT RAPID® and IDEXX LABORATORIES COLILERT-18®, page 18].
The manufacturing, storage and quality control of COLIKAT RAPID®
The manufacturing, storage and quality control of COLIKAT RAPID® is carried out in an ISO 9001 certified Xebios Diagnostics facility in compliance with EN ISO 11133 and Good Manufacturing Practice (GMP). The quality assurance testing of COLIKAT RAPID® takes place in EN ISO 17025 accredited laboratory that complies with all the requirements of EN ISO 11133 and EN ISO 9308-2.
Our quality management system includes broad testing procedures for sterility, stability, shelf life, and the microbiological performance & physical condition of our products. We trust only established partners which have proven to delivery according to our high quality standards for years for the sourcing of our raw materials and technical equipment.
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